Pekala Lab Protocols
- Bacterial Cultures General (PDF)
- BCA Pierce 2004 (PDF)
- Cell Culture (PDF)
- Cell Differentiation with MDI (PDF)
- Dialyzing Nuclear NER Lysates (PDF)
- DNA Gel (PDF)
- DNA Precipiation (PDF)
- GFP Flow Cytometry (PDF)
- IP Protocol (PSA) for RNA and Protein (PDF)
- IP Protocol Using Strepavidin Agarose (PDF)
- Making Freezedowns (PDF)
- Maxi Plasmid Prep (PDF)
- Mini Prep Following Maniatis Protocol (PDF)
- Mini Prep Protocol Using Qiagen Chemical Solutions (PDF)
- NePER 10 cm Plate (PDF)
- NE-PER 6-Well Protocol (PDF)
- NE-PER Nuclear and Cytoplasmic Extraction Reagents (PDF)
- Oil Red O Staining (PDF)
- PAGE Northern for microRNA (PDF)
- PCR with Taq Beads (PDF)
- Polysome Profile Protocol (PDF)
- PVDF Coomassie Protein Staining (PDF)
- Reverse Transcription using iScript (PDF)
- Reverse Transcription using Superscript III and oligo (PDF)
- RIP-Chip protocol (PDF)
- RNA Gel (PDF)
- RNA Isolation for 10cm Plates (PDF)
- Stripping blot by lowering pH (PDF)
- Transfection Protocol (PDF)
- Western Blot (PDF)